A Laboratory is in need of a Research Assistant II with experience working with genetically engineered mice and experience maintaining records for experiments.
Under supervision, identify, design, plan, and independently conduct assays & experiments, & assist in interpreting results.
Specific Examples: (A) Transfer cells or manipulate mice, then immunize mice, administer pharmaceutical test agent per protocol, collect sera, score mice for allergic lung disease; ultimately, recover sera, cells and tissues from the experimental subjects, and analyze these sera, tissues, and cells. (B) Prepare purified cells from mice, stimulate and grow in tissue culture, analyze cells and their products. (C) Interface with histology core and with collaborating partners
Ensure preparedness of project and laboratory for experiments and procedures.
Specific Examples: ( A) organize, breed, and determine genotypes of genetically engineered mice to be used in experiments. (B) Arrange for calibration and safety checks on Biological Safety Cabinets required for cell cultures in experiments. (C) Monitor supplies of compressed carbon dioxide and liquid nitrogen and arrange resupply in timely manner needed for the cell culture incubators and for storage of cell lines and samples. (D) Take charge of accurate and timely proposals of ordering via eProcurement, and then tracking receipt and proper distribution or storage of orders received
Provide appropriate figures with data, progress reports, and other written as well as oral documentation of experiments.
Specific Examples: (A) Quantify, tabulate and process ELISA and flow cytometry assay data measuring effects of pharmaceutical agent(s) on allergic lung inflammation. (B) Organize complex sets of data from flow cytometry, cell counts, and ELISA into Powerpoint slides. (C) Prepare and engage in individual (with Research Asst. Professor as well as with PI/lab head) as well as team reviews of written and verbal reports [several times a week with Res. Asst. Prof.; every two weeks for PI meetings; weekly presentation of ongoing flow of data at overall group meeting
Prepare, operate, and troubleshoot equipment in experimental procedures.
Specific Examples: (A) set up flow cytometer and adjust settings for use in analyses of fluorescent staining of cell suspensions in experiments. (B) Operate, and line up maintenance or repairs as needed, various centrifuges [desktop microcentrifuges; table-top and high-speed floor models; preparative ultracentrifuges.
Bachelor's or Masters Degree and 2 years experience.
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